May 2018: Congratulations to Johanna on publishing her first paper!
Clec9a-Mediated Ablation of Conventional Dendritic Cells Suggests a Lymphoid Path to Generating Dendritic Cells In Vivo.
In our most recent study, we set out to generate a DC depletion model based on deletion of Clec9a-expressing DC progenitors. To achieve this we crossed Clec9a-Cre mice to Rosa-lox-stop-lox-Diptheria Toxin reporter mice. We find that these mice show a complete loss of dendritic cell progenitors, but, to our surprise, do not exhibit a complete paucity of differentiated DCs. Specifically, cells with phenotypic characteristics of cDC2 populate the spleen and other periperhal organs of the mice. These cells are functionally and transcriptionally similar to cDCs in wild type control mice but show somatic rearrangements of Ig-heavy chain genes, characteristic of lymphoid origin cells. Our studies reveal a previously unappreciated developmental heterogeneity of cDCs and suggest that when myeloid cDC progenitors are impaired, the lymphoid lineage can generate cells with features of cDCs. We are currently investigating the origin and in vivo functions of the 'lymphoid' DC2 in further detail.
IRTG914: Student Retreat at the Villa Vigoni, Menaggio, Italy, September 25-28 2017
In September our PhD students were invited by the IRTG914 to a Joint Scientific Retreat with the CiM-IMPRS Münster. This provided a great opportunity to present progress and get an overview about projects of others in form of a talk or a poster in a fantastic scientific atmosphere. Keynote lectures by Jamey Marth (University of California, Santa Barbara, USA) and Emilio Hirsch (Torino) gave insight into their current research on inflammation at mucosal borders and scaffold functions of PI3Kgamma to fight airway infection, respectively. The trip was rounded up by a guided boat tour over lake como and a trip to Bellagio, where the beautiful landscape and Italian flair could be enjoyed.
July 2017: Maria joins the lab.
Maria Szente-Pasztoi is the newest member of our lab. Her project will focus on deciphering the functions of dendritic cells in early life. Maria is originally from Hungary but joins us from the Helmholtz Center in Braunschweig, Germany, where she did a first Postdoc in the lab of Jochen Hühn and studied dendritic cell development and function in the intestinal system.
First Lab Retreat: June 26-29 2017 Annaberg/Austria.
Our first lab retreat took place in the secluded Dachstein region of the Austrian Alps. Everyone presented an update on their projects. The relaxed environment of our holiday cottage allowed for lengthy and stimulating discussions leading to exciting ideas for current and future projects. Our scientific exchange was topped up by a scientific debate and some wonderful hiking in (not always) sunny weather.
SFB 914: Retreat in Obergurgl, Austria, March 19-22 2017.
Johanna and Barbara joined the crew of the SFB 914 - Trafficking of Immune Cells in Inflammation, Development and Disease (http://www.sfb914.med.uni-muenchen.de/index.html) for a stimulating scientific exchange in Obergurgl, Austria. Great talks, great posters and a little time for skiing in the beautiful Austrian Alps.